Regulation of sprouty expression by PLC plus or minus and calcium-dependent signals

• Published in: Biochemical and biophysical research communications Vol. 323; no. 3; pp. 1040 - 1047
• Main Authors: Abe, M, Naski, M C
• Format: Journal Article
• Language: English
• Published: 22.10.2004
• ISSN: 0006-291X
• DOI: 10.1016/j.bbrc.2004.08.198

Sprouty, an essential antagonist of fibroblast growth factor receptor signaling, is induced following fibroblast growth factor receptor activation. The signaling pathways that induce sprouty have been incompletely characterized. However, studies show that MAP kinase signaling stimulates sprouty induction in various cell lines. Here we report that activation of sprouty expression by basic fibroblast growth factor required phospholipase C plus or minus (PLC plus or minus ) and calcium-dependent signaling. We showed that the induction of sprouty was inhibited by chelation of intracellular or extracellular calcium and that a fibroblast growth factor receptor deficient for PLC plus or minus signaling only weakly induced sprouty expression. Additionally, inhibition of PLC plus or minus with a pharmacological antagonist repressed the induction of sprouty by basic fibroblast growth factor. These findings indicate that calcium-dependent signaling regulates sprouty expression and that PLC plus or minus is vital for this process. This pathway of sprouty induction may be critical at sites such as limb bud mesenchyme where MAP kinases are inactive.