Involvement of the yeast metacaspase Yca1 in ubp10-programmed cell death

UBP10 encodes a deubiquitinating enzyme of Saccharomyces cerevisiae. Its inactivation results in a complex phenotype characterized by a subpopulation of cells that exhibits the typical cellular markers of apoptosis. Here, we show that additional deletion of YCA1, coding for the yeast metacaspase, su...

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Bibliographic Details
Published inFEMS yeast research Vol. 5; no. 2; pp. 141 - 147
Main Authors Bettiga, M, Calzari, L, Orlandi, I, Alberghina, L, Vai, M
Format Journal Article
LanguageEnglish
Published 01.11.2004
Subjects
Saccharomyces cerevisiae
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Summary:UBP10 encodes a deubiquitinating enzyme of Saccharomyces cerevisiae. Its inactivation results in a complex phenotype characterized by a subpopulation of cells that exhibits the typical cellular markers of apoptosis. Here, we show that additional deletion of YCA1, coding for the yeast metacaspase, suppressed the ubp10 disruptant phenotype. Moreover, YCA1 overexpression, without any external stimulus, had a detrimental effect on growth and viability of ubp10 cells accompanied by an increase of apoptotic cells. This response was completely abrogated by ascorbic acid addition. We also observed that cells lacking UBP10 had an endogenous caspase activity, revealed by incubation in vivo with FITC-labeled VAD-fmk. All these results argue in favour of an involvement of the yeast metacaspase in the active cell death triggered by loss of UBP10 function.
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ISSN:1567-1356
DOI:10.1016/j.femsyr.2004.07.005