Detection of begomovirus in different varieties of soybean in Sinaloa, Mexico

Soybean (Glycine max, Merr.) is an alternative crop during the summer in Sinaloa, a northern state of Mexico. Over the last four years, symptoms of yellowing, curled leaves and stunting have been observed on soybean plantings. Over 200 samples from symptomatic soybean plants were collected and analy...

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Bibliographic Details
Published inPhytopathology Vol. 96; no. 6; p. S77
Main Authors Mendez-Lozano, J, Perea-Araujo, L L, Mauricio-Castillo, A, Ruelas-Ayala, R D, Valenzuela-Garcia, P, Leyva-Lopez, N E, Arguello-Astorga, G R
Format Journal Article
LanguageEnglish
Published 01.06.2006
Subjects
Begomovirus
Glycine max
Pepper golden mosaic virus
Pepper huasteco yellow vein virus
Rhynchosia golden mosaic virus
Mexico
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Summary:Soybean (Glycine max, Merr.) is an alternative crop during the summer in Sinaloa, a northern state of Mexico. Over the last four years, symptoms of yellowing, curled leaves and stunting have been observed on soybean plantings. Over 200 samples from symptomatic soybean plants were collected and analyzed for the presence of begomoviruses by DNA hybridization, using the coat protein gene of Pepper huasteco yellow vein virus (PHYVV) as a probe. 40 soybean samples were subsequently used for polymerase chain reaction (PCR) amplification using the degenerate primers pMot-CP (1), pRep-DGR and pCP70-Mot (2) and specific primers for PHYVV and PepGMV. PCR products were cloned into pGEM-T Easy vector (Promega, Madison, WI) and sequenced. The amplified viral DNA was compared with DNA sequences available at NCBI database using Blast. The highest sequence similarity was obtained with Pepper golden mosaic virus and Rhynchosia golden mosaic virus.
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ISSN:0031-949X