Isolation, description, and identification of bacteria associated with diseased coffee and avocado from Costa Rica

The interaction of endophytic bacteria with citrus and citrus variegated chlorosis (CVC) was previously analyzed. X. fastidiosa is the cause of crespera of coffee and is associated with CVC-like symptoms in avocado. Bacteria were obtained during attempts to isolate X. faslidiosa from symptomatic cof...

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Published inPhytopathology Vol. 96; no. 6; p. S162
Main Authors Chacon, C, Montero-Astua, M, Hartung, J S, Li, W B, Garita, L, Rivera, C
Format Journal Article
LanguageEnglish
Published 01.06.2006
Subjects
Citrus
Enterobacter
Moraxella
Pantoea agglomerans
Persea americana
Pseudomonas
Costa Rica
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Summary:The interaction of endophytic bacteria with citrus and citrus variegated chlorosis (CVC) was previously analyzed. X. fastidiosa is the cause of crespera of coffee and is associated with CVC-like symptoms in avocado. Bacteria were obtained during attempts to isolate X. faslidiosa from symptomatic coffee and avocado. Coffee strains and some avocado strains were characterized by colony morphology, Gram stain, biochemical assays, and electron microscopy. M. mesophilicum was isolated from symptomatic avocados and 10 trees were PCR positive. Pseudomonas syringae-glycinea, Pantoea agglomerans, C. flaccumfaciens-betae/oortii, Moraxella nonliquefaciens, and Enterobacter intermedius were isolated and identified by fatty-acid methyl ester analysis. Plans are to confirm them as endophytes, determine their identity/frequency, and determine an interaction with X. fastidiosa in crespera in the two diseases.
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ISSN:0031-949X