Molecular cloning and function analysis of phosphatidyl inositol 3 kinase p85 alpha subunit gene NlPIK3R1 in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

• Published in: K'un ch'ung hsueh pao Vol. 58; no. 5; pp. 487 - 495
• Main Authors: LU, Chao-Feng, HAO, Pei-Ying, MA, Yan, ZHU, Jia-Jun, FENG, Ya-Lin, YU, Xiao-Ping
• Format: Journal Article
• Language: Chinese
• Published: 01.05.2015
• Subjects: Delphacidae; Hemiptera; Nilaparvata lugens
• ISSN: 0454-6296
• DOI: 10.16380/j.kcxb.2015.05.003

This study aims to study the function of phosphatidylinositol 3-kinase in the rice brown planthopper, Nilaparvata lugens. The full-length cDNA of NlPIK3R1 gene was cloned by rapid-amplification of cDNA ends (RACE) according to its gene sequence information available in N. lugens transcriptome, and this gene encodes the p85 alpha subunit of PI3K (PI3K p85 alpha ), with the GenBank accession number of KP635379. Then the real-time quantitative PCR and RNAi by feeding dsNlPIK3R1 to N. lugens adults were conducted to explore the expression pattern and the function of NlPIK3R1, respectively. The real-time quantitative PCR results showed that the NlPIK3R1 gene was slightly expressed in nymphs and male adults of N. lugens, but strongly expressed in gravid female adults. RNAi results showed that the expression of NlPIK3R1 was inhibited in the two dsNlPIK3R1 treatment groups (fed with 0. 1 and 0.5 mu g/ mu L dsNlPIK3R1, respectively), especially in the high concentration treatment group. Feeding of dsNlPIK3R1 led to th