Recognition of tRNA super(Phe) by phenylalanyl-tRNA synthetase of Thermus thermophilus

The tRNA super(Phe) nucleotides required for recognition by phenylalanyl-tRNA synthetase of Thermus thermophilus have been determined using Escherichia coli tRNA super(Phe) transcripts with various mutations. The anticodon nucleotides are shown to be the most important recognition elements. The disc...

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Published inEuropean journal of biochemistry Vol. 234; no. 3; pp. 897 - 902
Main Authors Moor, NA, Ankilova, V N, Lavrik, OI
Format Journal Article
LanguageEnglish
Published 01.01.1995
Subjects
Escherichia coli
Saccharomyces cerevisiae
Thermus thermophilus
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Summary:The tRNA super(Phe) nucleotides required for recognition by phenylalanyl-tRNA synthetase of Thermus thermophilus have been determined using Escherichia coli tRNA super(Phe) transcripts with various mutations. The anticodon nucleotides are shown to be the most important recognition elements. The discriminator nucleotide, A73, involved in the recognition set of yeast, E. coli and human phenylalanyl-tRNA synthetases contributes only slightly to tRNA super(Phe) recognition by Th. thermophilus phenylalanyl-tRNA synthetase. Nucleotide 20 and some tertiary nucleotides, including the conserved G19 - C56 base pair, are proposed to participate in stabilization of the precise tRNA conformation required for efficient aminoacylation. The role of the 3'-CCA terminus, common to all tRNAs, in the specific interaction of tRNA with phenylalanyl-tRNA synthetase is discussed.
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ISSN:0014-2956