Conserved and Variable Functions of the capital sigma super(E) Stress Response in Related Genomes

• Published in: PLoS biology Vol. 4; no. 1
• Main Authors: Rhodius, Virgil A, Suh, Won Chul, Nonaka, Gen, West, Joyce, Gross, Carol A
• Format: Journal Article
• Language: English
• Published: 01.01.2006
• Subjects: Escherichia coli
• ISSN: 1544-9173; 1545-7885
• DOI: 10.1371/journal.pbio.0040002

Bacteria often cope with environmental stress by inducing alternative sigma ( capital sigma ) factors, which direct RNA polymerase to specific promoters, thereby inducing a set of genes called a regulon to combat the stress. To understand the conserved and organism-specific functions of each capital sigma , it is necessary to be able to predict their promoters, so that their regulons can be followed across species. However, the variability of promoter sequences and motif spacing makes their prediction difficult. We developed and validated an accurate promoter prediction model for Escherichia coli capital sigma super(E), which enabled us to predict a total of 89 unique capital sigma super(E)-controlled transcription units in E. coli K-12 and eight related genomes. capital sigma super(E) controls the envelope stress response in E. coli K-12. The portion of the regulon conserved across genomes is functionally coherent, ensuring the synthesis, assembly, and homeostasis of lipopolysaccharide and outer membrane porins, the key constituents of the outer membrane of Gram-negative bacteria. The larger variable portion is predicted to perform pathogenesis-associated functions, suggesting that capital sigma super(E) provides organism-specific functions necessary for optimal host interaction. The success of our promoter prediction model for capital sigma super(E) suggests that it will be applicable for the prediction of promoter elements for many alternative capital sigma factors.