Cloning and characterization of the Bacillus subtilis gene encoding a repressor of the biotin operon

The Bacillus subtilis gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degree on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to...

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Published inJournal of bacteriology Vol. 177; no. 9; pp. 2572 - 2575
Main Authors Bower, S, Perkins, J, Yocum, R R, Serror, P, Sorokin, A, Rahaim, P, Howitt, CL, Prasad, N, Ehrlich, S D, Pero, J
Format Journal Article
LanguageEnglish
Published 01.01.1995
Subjects
Bacillus subtilis
Escherichia coli
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Summary:The Bacillus subtilis gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degree on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to deregulation of biotin synthesis alter single amino acids in the amino-terminal end of the protein. The amino-terminal region that is affected by these three birA mutations shows sequence similarity to the helix-turn-helix DNA binding motif previously identified in E. coli BirA protein. B. subtilis BirA protein also possesses biotin-protein ligase activity, as judged by its ability to complement a conditional lethal birA mutant of E. coli.
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ISSN:0021-9193