IgE super(+) cells in the peripheral blood of atopic, nonatopic, and bee venom-hypersensitive individuals exhibit the phenotype of highly differentiated B cells
We have analyzed IgE super(+) cells in peripheral blood of atopic donors, donors hypersensitive to bee venom, and nonatopic control donors with two- and three-color flow cytometry. Although the percentage of IgE super(+) cells varied among these groups, the overall phenotypic patterns were similar....
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Published in | Journal of allergy and clinical immunology Vol. 95; no. 2; pp. 587 - 596 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
01.01.1995
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Subjects | |
Online Access | Get full text |
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Summary: | We have analyzed IgE super(+) cells in peripheral blood of atopic donors, donors hypersensitive to bee venom, and nonatopic control donors with two- and three-color flow cytometry. Although the percentage of IgE super(+) cells varied among these groups, the overall phenotypic patterns were similar. Most IgE super(+) cells do not display typical B-cell markers, such as CD19, CD20, and CD21. A significant proportion of these cells strain for CD38, indicating that they are more differentiated. IgE super(+) cells express Fc gamma RII and CD45RO, an isoform associated with an advanced level of differentiation. The majority of IgE super(+) cells do not coexpress other surface immunoglobulin isotypes. In the case of bee venom-hypersensitive donors, we have been able to identify a small population of IgE super(+) cells with a specificity for phospholipase A2, a major immunogenic component of bee venom. The phospholipase A2 super(+) cells display a phenotype similar to that of the IgE super(+) cells. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
ISSN: | 0091-6749 |