Solubilized rabbit striatal A sub(2a)-adenosine receptors: Stability and antagonist binding

The A sub(2a)-adenosine binding subunit from rabbit striatal membranes was solubilized using 1% 3-[(3-cholamidopropyl)dimethylammonio] 1-propanesulfonate and was characterized using the antagonist radioligand [ super(3)H]8-[4-(((2-aminoethyl)amino)carbonyl) methyl)oxy)phenyl]-1,3 -dipropylxanthine (...

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Published inArchives of biochemistry and biophysics Vol. 305; no. 2; pp. 611 - 617
Main Authors Ji, Xiao-Duo, Jacobson, KA
Format Journal Article
LanguageEnglish
Published 01.01.1993
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Summary:The A sub(2a)-adenosine binding subunit from rabbit striatal membranes was solubilized using 1% 3-[(3-cholamidopropyl)dimethylammonio] 1-propanesulfonate and was characterized using the antagonist radioligand [ super(3)H]8-[4-(((2-aminoethyl)amino)carbonyl) methyl)oxy)phenyl]-1,3 -dipropylxanthine (XAC). Xanthine inhibitors displaced the specific binding of the adenosine antagonist [ super(3)H]XAC. Preincubation with 100 mu M GTP converted the high affinity binding to low affinity, suggesting that receptor and G-protein are dissociated by the guanine nucleotide.
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SourceType-Scholarly Journals-1
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ISSN:0003-9861