Purification of a cytochrome b containing H sub(2):Heterodisulfide oxidoreductase complex from membranes of Methanosarcina barkeri

• Published in: European journal of biochemistry Vol. 213; no. 1; pp. 529 - 535
• Main Authors: Heiden, S, Hedderich, R, Setzke, E, Thauer, R K
• Format: Journal Article
• Language: English
• Published: 01.01.1993
• Subjects: Methanosarcina barkeri
• ISSN: 0014-2956

The reduction of CoM-S-S-HTP, the heterodisulfide of coenzyme M (H-S-CoM) and N-7-mercaptoheptanoyl-threonine phosphate (H-S-HTP), with H sub(2) is an energy-conserving step in methanogenic archaea. In Methanosarcina barkeri) this reaction is catalyzed by a membrane-bound multienzyme complex, designated H sub(2):heterodisulfide oxidoreductase complex, which was purified to apparent homogeneity. The preparation was found to be composed of nine polypeptides of apparent molecular masses 46 kDa, 39 kDa, 28 kDa, 25 kDa, 23 kDa, 21 kDa, 20 kDa, 16 kDa, and 15 kDa and to contain 3.2 nmol cytochrome b, 70 to 80 nmol non-heme iron and acid-labile sulfur, 5 nmol Ni, and 0.6 nmol FAD per mg protein. The 23 kDa polypeptide possessed heme-derived peroxidase activity indicating that this polypeptide is the cytochrome b. The purified H sub(2):heterodisulfide oxidoreductase complex catalyzed the reduction of CoM-S-S-HTP with H sub(2) at a specific activity of 6 U/mg protein (1 U = 1 mu mol / min), the reduction of benzylviologen with H sub(2) at a specific activity of 66 U/mg protein and the reduction of CoM-S-S-HTP with reduced benzylviologen at a specific activity of 24 U/mg protein. The complex did not mediate the reduction of coenzyme F sub(420) with H sub(2) nor the oxidation of reduced coenzyme F sub(420) with CoM-S-S-HTP. The reduced cytochrome b in the enzyme complex could be oxidized by CoM-S-S-HTP and re-reduced by H sub(2). The specific rates of cytochrome oxidation and reduction were too high to be resolved under our experimental conditions. The findings suggest that the H sub(2):heterodisulfide oxidoreductase complex is composed of a F sub(420)-non-reducing hydrogenase, a cytochrome b and heterodisulfide reductase and that cytochrome b is a redox carrier in the electron transport chain involved in CoM-S-S-HTP reduction with H sub(2).