Evaluation of fractional photothermolysis effect in a mouse model using nonlinear optical microscopy

Fractional photothermolysis (FP) induces discrete columns of photothermal damage in skin dermis, thereby promoting collagen regeneration. This technique has been widely used for treating wrinkles, sun damage, and scar. In this study, we evaluate the potential of multiphoton microscopy as a noninvasi...

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Bibliographic Details
Published inJournal of biomedical optics Vol. 19; no. 7
Main Authors Guo, Han Wen, Tseng, Te-Yu, Dong, Chen-Yuan, Tsai, Tsung-Hua
Format Journal Article
LanguageEnglish
Published 01.07.2014
Subjects
Collagens
Damage
Filled plastics
Monitoring
Nonlinearity
Optical microscopy
Regeneration
Second harmonic generation
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Summary:Fractional photothermolysis (FP) induces discrete columns of photothermal damage in skin dermis, thereby promoting collagen regeneration. This technique has been widely used for treating wrinkles, sun damage, and scar. In this study, we evaluate the potential of multiphoton microscopy as a noninvasive imaging modality for the monitoring of skin rejuvenation following FP treatment. The dorsal skin of a nude mouse underwent FP treatment in order to induce microthermal zones (MTZs). We evaluated the effect of FP on skin remodeling at 7 and 14 days after treatment. Corresponding histology was performed for comparison. After 14 days of FP treatment at 10 mJ, the second harmonic generation signal recovered faster than the skin treated with 30 mJ, indicating a more rapid regeneration of dermal collagen at 10 mJ. Our results indicate that nonlinear optical microscopy is effective in detecting the damaged areas of MTZ and monitoring collagen regeneration following FP treatment.
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ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.19.7.075005