Inhibition study of human placental aromatase with 3-deoxy- and 6-oxo-steroids using (1 beta - super(3)H)16 alpha -hydroxyandrostenedione as a substrate: A comparison to that using (1 beta - super(3)H)androstenedione
Inhibition of aromatase activity in human placental microsomes with 3-deoxy- (1, 2, 4, 6 and 8), 6-oxo- (3, 5 and 9), and 16 alpha -bromo- (7) steroids was studied using (1 beta - super(3)H)16 alpha -hydroxyandrostenedione (16 alpha -OHAD) as a substrate and compared to that with the conventional su...
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| Published in | The Journal of steroid biochemistry and molecular biology Vol. 44; no. 2; pp. 199 - 201 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
01.01.1993
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| Online Access | Get full text |
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| Summary: | Inhibition of aromatase activity in human placental microsomes with 3-deoxy- (1, 2, 4, 6 and 8), 6-oxo- (3, 5 and 9), and 16 alpha -bromo- (7) steroids was studied using (1 beta - super(3)H)16 alpha -hydroxyandrostenedione (16 alpha -OHAD) as a substrate and compared to that with the conventional substrate, (1 beta - super(3)H)androstenedione. All the steroids inhibited both 16 alpha -OHAD and (1 beta - super(3)H)androstenedione aromatization in a competitive manner. Based on K sub(i)/K sub(m) ratios obtained in both series of experiments, their relative inhibitory activities in the series with 16 alpha -OHAD were not necessarily identical with those in the other series. |
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| Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
| ISSN: | 0960-0760 |