Functional difference between SERCA2a and SERCA2b Ca super(2+) pumps and their modulation by phospholamban

COS 1 cells were transfected with full-length pig stomach sarcoplasmic/endoplasmic reticulum Ca super(2+) pump (SERCA)2a or SERCA2b cDNA. Ca super(2+) uptake by microsomes from transfected cells revealed that the Ca super(2+) affinity of the SERCA2b Ca super(2+) pump (K sub(0.5) 0.17 plus or minus 0...

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Published inBiochemical journal Vol. 286; no. 2; pp. 591 - 596
Main Authors Verboomen, H, Wuytack, F, De Smedt, H, Himpens, B, Casteels, R
Format Journal Article
LanguageEnglish
Published 01.01.1992
Subjects
Ca super(2+)-transporting ATPase
effects on
endoplasmic reticulum
isoenzymes
phospholamban
pigs
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Summary:COS 1 cells were transfected with full-length pig stomach sarcoplasmic/endoplasmic reticulum Ca super(2+) pump (SERCA)2a or SERCA2b cDNA. Ca super(2+) uptake by microsomes from transfected cells revealed that the Ca super(2+) affinity of the SERCA2b Ca super(2+) pump (K sub(0.5) 0.17 plus or minus 0.01 mu M) was higher than that of the SERCA2a Ca super(2+) pump (K sub(0.5) 0.31 plus or minus 0.02 mu M). Thapsigargin-sensitivity was found to be identical for the two isoforms. The Ca super(2+) affinity of both the SERCA2a and SERCA2b Ca super(2+) pumps was decreased by a factor of two when they were co-expressed with phospholamban.
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ISSN:0264-6021