Uniform super(15)N labeling of a fungal peptide: The structure and dynamics of an alamethicin by super(15)N and super(1)H NMR spectroscopy

An alamethicin, secreted by the fungus Trichoderma viride and containing a glutamine at position 18 instead of the usual glutamic acid, has been uniformly labeled with super(15)N and purified. The proton NMR spectrum of the peptide dissolved in methanol was assigned using correlation spectroscopies...

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Bibliographic Details
Published inBiochemistry (Easton) Vol. 31; no. 12; pp. 3135 - 3143
Main Authors Adelinda, A, Yee, A, O'Neil, JDJ
Format Journal Article
LanguageEnglish
Published 01.01.1992
Subjects
alamethicin
N.M.R
structure
Trichoderma viride
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Summary:An alamethicin, secreted by the fungus Trichoderma viride and containing a glutamine at position 18 instead of the usual glutamic acid, has been uniformly labeled with super(15)N and purified. The proton NMR spectrum of the peptide dissolved in methanol was assigned using correlation spectroscopies and nuclear Overhauser enhancements measured in the rotating frame.
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SourceType-Scholarly Journals-1
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ISSN:0006-2960