Percutaneous absorption of ( super(14)C)DDT and ( super(14)C) benzo(a)pyrene from soil

• Published in: Fundamental and applied toxicology Vol. 15; no. 3; pp. 510 - 516
• Main Authors: Wester, R C, Maibach, H I, Bucks, DAW, Sedik, L, Melendres, J, Liao, Cheng, CiZio, S
• Format: Journal Article
• Language: English
• Published: 01.01.1990
• ISSN: 0272-0590

The objective was to determine percutaneous absorption of DDT and benzo(a)pyrene in vitro and in vivo from soil into and through skin. Soil (Yolo County 65-California-57-8; 26% sand, 26% clay, 48% silt) was passed through 10-, 20-, and 48-mesh sieves. Soil then retained by 80-mesh was mixed with ( super(14)C)-labeled chemical at 10 ppm. Acetone solutions at 10 ppm were prepared for comparative analysis. Human cadaver skin was dermatomed to 500 mu m and used in glass diffusion cells with human plasma as the receptor fluid (3 ml/hr flow rate) for a 24-hr skin application time. With acetone vehicle, DDT (18.1%) readily penetrated into human skin. Significantly less DDT (1.0%) penetrated into human skin from soil. DDT would not partition from human skin into human plasma in the receptor phase (<0.1%). With acetone vehicle, benzo(a)pyrene (23.7%) readily penetrated into human skin. Significantly less benzo(a)pyrene (1.4%) penetrated into human skin from soil. Benzo(a)pyrene would not partition from human skin into human plasma in the receptor phase (<0.1%).