Gene pho-2 codes for the multiple active forms of P sub(i)-repressible alkaline phosphatase in the mould Neurospora crassa

The mycelial P sub(i)-repressible alkaline phosphatase of the wild-type strain 74A of Neurospora crassa was separated into at least ten isoforms by isoelectric focusing. The components visualized by activity with sodium alpha -naphthyl phosphate as the substrate were predominantly acidic proteins wi...

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Published inWorld journal of microbiology & biotechnology Vol. 13; no. 6; pp. 609 - 611
Main Authors Thedei, G Jr, Nozawa, SR, Simoes, AL, Rossi, A
Format Journal Article
LanguageEnglish
Published 01.11.1997
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Summary:The mycelial P sub(i)-repressible alkaline phosphatase of the wild-type strain 74A of Neurospora crassa was separated into at least ten isoforms by isoelectric focusing. The components visualized by activity with sodium alpha -naphthyl phosphate as the substrate were predominantly acidic proteins with isoelectric points ranging from pH 4.5 to 7.6. The number of these isoforms was a function of growth pH. Strain pho-2A did not produce active P sub(i)-repressible alkaline phosphatase (the pho-2 gene codes for its amino acid sequence), which gives an indication that these isoforms are encoded by the same structural gene.
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ISSN:0959-3993