Transcriptional enhancer related DNA sequences: Anomalous super(1)H NMR NOE crosspeaks

We examine three additional biologically significant 17 base pair duplexes containing GTG/CAC triplets: (1) a sequence from the mouse heavy chain immunoglobulin enhancer, (2) a sequence from the critical core of the Simian Virus 40 (SV40) enhancer, and (3) a sequence from pBR322 plasmid used as cont...

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Published inNucleic acids research Vol. 20; no. 3; pp. 525 - 532
Main Authors Donlan, ME, Lu, Ponzy
Format Journal Article
LanguageEnglish
Published 01.01.1992
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Summary:We examine three additional biologically significant 17 base pair duplexes containing GTG/CAC triplets: (1) a sequence from the mouse heavy chain immunoglobulin enhancer, (2) a sequence from the critical core of the Simian Virus 40 (SV40) enhancer, and (3) a sequence from pBR322 plasmid used as control for experiments with the SV40 DNA sequences. The super(1)H NMR resonance assignment for nearly all the nonexchangeable protons for both eukaryotic enhancer duplexes with the exception of the H5'/H5" protons was accomplished to use for structural analysis of these duplexes. The data presented show several NOE's associated with the GTG/CAC triplets which suggest structural variation from uniform B-DNA. Anomalous broad crosspeaks for the fixed thymine methyl to its own H6 proton in combination wit the imino proton kinetics associated with these triplets reinforces the original observation of a sequence dependent dynamic variation.
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ISSN:0305-1048