Recovery of homogeneous and functional beta sub(2)-adrenergic receptors from extracellular baculovirus particles
Expression in baculovirus-infected insect cells allows sufficient production of G-protein coupled receptor for structural studies. An important drawback of this expression system comes from the presence of unprocessed and biologically inactive receptors that have to be eliminated during receptor pur...
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Published in | Nature biotechnology Vol. 15; no. 12; pp. 1300 - 1312 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.11.1997
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Online Access | Get full text |
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Summary: | Expression in baculovirus-infected insect cells allows sufficient production of G-protein coupled receptor for structural studies. An important drawback of this expression system comes from the presence of unprocessed and biologically inactive receptors that have to be eliminated during receptor purification steps. We show that viral particles released from Sf9 cells infected with a recombinant baculovirus coding for the human beta sub(2)-adrenergic receptor ( beta sub(2)AR) cDNA contain glycosylated and biologically active beta sub(2)AR. In addition, post-translational modifications known to modulate receptor activity were found to occur in these particles. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
ISSN: | 1087-0156 |