A New Ochratoxin A Biodegradation Strategy Using Cupriavidus basilensis [Odblac]r16 Strain: e109817

• Published in: PloS one Vol. 9; no. 10
• Main Authors: Ferenczi, Szilamer, Cserhati, Matyas, Krifaton, Csilla, Szoboszlay, Sandor, Kukolya, Jozsef, Szoke, Zsuzsanna, Koszegi, Balazs, Albert, Mihaly, Barna, Terez, Mezes, Miklos
• Format: Journal Article
• Language: English
• Published: 01.10.2014
• ISSN: 1932-6203
• DOI: 10.1371/journal.pone.0109817

Ochratoxin-A (OTA) is a mycotoxin with possibly carcinogenic and nephrotoxic effects in humans and animals. OTA is often found as a contaminant in agricultural commodities. The aim of the present work was to evaluate OTA-degrading and detoxifying potential of Cupriavidus basilensis [Odblac]R16 strain. In vivo administration of OTA in CD1 male mice (1 or 10 mg/kg body weight for 72 hours or 0.5 mg/kg body weight for 21 days) resulted in significant elevation of OTA levels in the blood, histopathological alterations- and transcriptional changes in OTA-dependent genes (annexinA2, clusterin, sulphotransferase and gadd45 and gadd153) in the renal cortex. These OTA-induced changes were not seen in animals that have been treated with culture supernatants in which OTA was incubated with Cupriavidus basilensis [Odblac]R16 strain for 5 days. HPLC and ELISA methods identified ochratoxin alpha as the major metabolite of OTA in Cupriavidus basilensis [Odblac]R16 cultures, which is not toxic in vivo. This study has demonstrated that Cupriavidus basilensis [Odblac]R16 efficiently degrade OTA without producing toxic adventitious metabolites.