Human interferon omega 1: Isolation of the gene, expression in Chinese hamster ovary cells and characterization of the recombinant protein

A gene encoding human interferon omega -1 (IFN- omega 1) was isolated from a cosmid library, sequenced and expressed in Chinese hamster ovary (CHO) cells under the control of an SV40-derived promoter/enhancer sequence. Amino acid sequencing showed that only about 40% of the molecules have the expect...

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Published inBiochimica et biophysica acta, Protein structure and molecular enzymology Vol. 1089; no. 2; pp. 167 - 174
Main Authors Adolf, G R, Fruehbeis, B, Hauptmann, R, Kalsner, I, Maurer-Fogy, I, Ostermann, E, Patzelt, E, Schwendenwein, R, Sommergruber, W, Zoephel, A
Format Journal Article
LanguageEnglish
Published 01.01.1991
Subjects
genes
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Summary:A gene encoding human interferon omega -1 (IFN- omega 1) was isolated from a cosmid library, sequenced and expressed in Chinese hamster ovary (CHO) cells under the control of an SV40-derived promoter/enhancer sequence. Amino acid sequencing showed that only about 40% of the molecules have the expected N-terminus, whereas the others carry two additional amino acids derived from the signal sequence. C-terminal amino acid sequencing using carboxypeptidase P demonstrated that the smaller form of the protein lacks nine amino acids.
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ISSN:0167-4838