Evidence for involvement of tyrosine in estradiol binding by rat uterus estrogen receptor

The possibility of tyrosine involvement in steroid binding by rat uterus estrogen receptor (rER) was investigated. Chemical modification of rER with reagents such as tetranitromethane (TNM) and N-acetylimidazole (NAcI) inhibited estradiol binding. Steroid binding was inhibited to a greater extent at...

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Published inJournal of steroid biochemistry Vol. 38; no. 2; pp. 135 - 139
Main Authors Koffman, B, Modarress, K J, Beckerman, T, Bashirelahi, N
Format Journal Article
LanguageEnglish
Published 01.01.1991
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Summary:The possibility of tyrosine involvement in steroid binding by rat uterus estrogen receptor (rER) was investigated. Chemical modification of rER with reagents such as tetranitromethane (TNM) and N-acetylimidazole (NAcI) inhibited estradiol binding. Steroid binding was inhibited to a greater extent at pH 8 than at pH 6, indicating the participation of tyrosine (TNM has increasing affinity for cysteine over tyrosine at pH 6). Inhibition patterns remained similar for incubations at 0-4 degree C or 37 degree C. NAcI inhibited rER steroid binding at 37 degree C, but not at 0-4 degree C. Hydroxylamine incubated in the presence of rER and NAcI appeared to reverse this inhibition.
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ISSN:0022-4731