Analysis of Krueppel control elements reveals that localized expression results from the interaction of multiple subelements

The Drosophila gap gene Krueppel (Kr) displays a complex spatiotemporal pattern of expression during embryogenesis. Using P-element transformation experiments, we demonstrate that control elements guiding Kr expression in the central or in the anterior domain at the blastoderm stage are each compose...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 88; no. 13; pp. 5912 - 5916
Main Authors Jacob, Y, Sather, S, Martin, J R, Ollo, R
Format Journal Article
LanguageEnglish
Published 01.01.1991
Subjects
Diptera
Drosophilidae
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Summary:The Drosophila gap gene Krueppel (Kr) displays a complex spatiotemporal pattern of expression during embryogenesis. Using P-element transformation experiments, we demonstrate that control elements guiding Kr expression in the central or in the anterior domain at the blastoderm stage are each composed of multiple subelements that interact synergistically. We provide evidence that bicoid (bcd) and hunchback (hb) gene products, as well as at least one other activator, are needed to activate Kr expression in the central domain. We localize and describe regulatory elements within the 4.1-kilobase region proximal to the Kr promoter that are responsible for expression in the ectoderm, mesoderm, amnioserosa, and nervous system.
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ISSN:0027-8424