5'-S-(2-aminoethyl)-N super(6)-(4-nitrobenzyl)-5'-thioadenosine (SAENTA), a novel ligand with high affinity for polypeptides associated with nucleoside transport. Partial purification of the nitrobenzylthioinosine-binding protein of pig erythrocytes by affinity chromatography

Derivatives of N super(6)-(4-aminobenzyl)adenosine (substituted at the aminobenzyl group) and 5'-linked derivatives of N super(6)-(4-nitrobenzyl)adenosine were evaluated as inhibitors of site-specific binding of ( super(3)H)-nitrobenzylthioinosine (NBMPR) to pig erythrocyte membranes. The resul...

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Published inBiochemical journal Vol. 270; no. 3; pp. 605 - 614
Main Authors Agbanyo, FR, Vijayalakshmi, D, Craik, J D, Gati, W P, McAdam, D P, Asakura, J-I, Robins, MJ, Paterson, ARP, Cass, CE
Format Journal Article
LanguageEnglish
Published 01.01.1990
Subjects
membrane proteins
nitrobenzylthioinosine-binding protein
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Summary:Derivatives of N super(6)-(4-aminobenzyl)adenosine (substituted at the aminobenzyl group) and 5'-linked derivatives of N super(6)-(4-nitrobenzyl)adenosine were evaluated as inhibitors of site-specific binding of ( super(3)H)-nitrobenzylthioinosine (NBMPR) to pig erythrocyte membranes. The results demonstrate that polypeptide components of NBMPR-sensitive nucleoside-transport systems may be partly purified by affinity chromatography using gel media bearing SAENTA groups.
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ISSN:0264-6021