Regulation of spo0H, a gene coding for the Bacillus subtilis sigma super(H) factor

The Bacillus spo0H gene codes for sigma super(H), which, as part of the RNA polymerase holoenzyme E sigma super(H), is responsible for the transcription of several genes which are expressed at the beginning of the sporulation process. In this communication, we examined the regulation of the spo0H ge...

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Published inJournal of bacteriology Vol. 173; no. 2; pp. 521 - 529
Main Authors Weir, J, Predich, M, Dubnau, E, Nair, G, Smith, I
Format Journal Article
LanguageEnglish
Published 01.01.1991
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Summary:The Bacillus spo0H gene codes for sigma super(H), which, as part of the RNA polymerase holoenzyme E sigma super(H), is responsible for the transcription of several genes which are expressed at the beginning of the sporulation process. In this communication, we examined the regulation of the spo0H gene of Bacillus subtilis) by using lacZ reporter gene assays, quantitative RNA determinations, and Western immunoassay. The studies indicate that spo0H is transcribed by the major vegetative RNA polymerase, E sigma super(A). spo0H RNA and sigma super(H) levels during growth are not identical to each other or to the pattern of expression of spoVG, a gene transcribed by E sigma super(H). This suggests that spo0H is regulated posttranscriptionally and also that factors in addition to sigma super(H) levels are involved in the expression of genes of the E sigma super(H) regulon.
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ISSN:0021-9193