Quantitative analysis of marine oils by capillary supercritical fluid chromatography

• Published in: Chromatographia Vol. 19; no. 11-12; pp. 697 - 705
• Main Authors: Staby, A, Borch-Jensen, C, Balchen, S, Mollerup, J
• Format: Journal Article
• Language: English
• Published: 01.01.1994
• Subjects: Marine
• ISSN: 0009-5893

Supercritical fluid chromatographic analysis methods have been employed in the examination of several marine oils for the group separation of free fatty acids, retinol, ergocalciferol, cholecalciferol, squalene, tocopherols, cholesterol, wax esters, diacylglycerols, cholesteryl esters, and triacylglycerols. The oils were derived from characteristic species including shark, seal, edible and trash fish. The supercritical fluid chromatography (SFC) method used for the separation of the liquids utilize carbon dioxide as the mobile phase, a non-polar capillary column, and flame ionization detection. The SFC methods have proved capable of making a considerable contribution to the continuing investigations into the structure and composition of marine oils. Furthermore SFC analyses, with their very simple sample preparation requirements, may serve as alternatives or supplements to the existing range of chromatographic and non-chromatographic analysis methods used in the examination of these oils.