Analytical method development and validation for the simultaneous estimation of Levamisole and Mebendazole in bulk & tablet formulation by RP-HPLC method

• Published in: Indian journal of research in pharmacy and biotechnology Vol. 2; no. 1; p. 952
• Main Authors: Rao, V Rama Koteswara, Agarwal, Nanda Kishore, Pavami, K Haritha, Kumar, B Prem, Mallikarjuna, R
• Format: Journal Article
• Language: English
• Published: 01.01.2014
• ISSN: 2321-5674; 2320-3471

A fast, robust and accurate RP-HPLC method was developed and validated for simultaneous determination of Levamisole and Mebendazole in tablets. The mobile phase was mixture of aqueous phosphate buffer with pH 5.2: Methanol: Acetonitrile, effluent flow rate monitored at 1.0 ml/min. the stationary phase was C18 column, zodiac 5 mu . The solutions of standard and the sample were prepared in methanol. The retention times was found to be 2.427min and 4.110min for Levamisole and Mebendazole respectively at 235nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of 150-210 mu g/ml. The percentage assay for Levamisole and Mebendazole were found to be 99.94% and 99.35%respectively. The LOD and LOQ were found to be0.0034 mu gm/ml and 0.00104 mu gm/ml for Levamisole, 90-210 mu gm/ml and 60-140 mu gm/ml for Mebendazole. The method was validated and it was found to be accurate, precise, linear and reproducible.