Queuosine deficient tRNA super(His) and tRNA super(Asp) from the spleens of young mice, erythroleukemic tumoral spleens and cultured friend cells

tRNAs were isolated from the spleens of young mice, erythroleukemic spleens and cultured Friend cells. Queuosine (Q) deficient tRNAs were labelled in their anticodon with radioactive guanine. tRNAs were then specifically aminoacylated. In both normal and tumoral spleen (TF-P10), tRNA super(His) was...

Full description

Saved in:
Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 109; no. 4; pp. 1140 - 1147
Main Authors Landin, R M, Petrissant, G
Format Journal Article
LanguageEnglish
Published 01.01.1982
Online AccessGet full text

Cover

More Information
Summary:tRNAs were isolated from the spleens of young mice, erythroleukemic spleens and cultured Friend cells. Queuosine (Q) deficient tRNAs were labelled in their anticodon with radioactive guanine. tRNAs were then specifically aminoacylated. In both normal and tumoral spleen (TF-P10), tRNA super(His) was the main guanine containing (G) isoacceptor as shown by RPC-5 chromatography. In vitro, the tumor derived cell line (TF-P10c) retained the G-tRNA super(His) species while Friend cell line, clone 707 (FLC), did not. A common feature found in both cultured cell lines was the high level of G-tRNA super(Asp). The meaning of the relative abundance of Q-deficient tRNA super(His) and of tRNA super(Asp) observed in transformed cells of erythroid origin is discussed.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
content type line 23
ObjectType-Feature-1
ISSN:0006-291X