Guanine Nucleotides do not Inhibit Tritiated Dopamine Agonist Binding to Bovine Striatal Membranes

The addition of GTP (50 mu M), MnCl sub(2) (1 mM) or EDTA (2 mM) had no effect on the affinity or capacity of bovine striatal plasma membranes for ( super(3)H)spiperone. However, GTP caused a decrease in the potency of dopamine as an inhibitor of ( super(3)H)spiperone binding under all conditions te...

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Bibliographic Details
Published inNeurochemical research Vol. 7; no. 7; pp. 831 - 837
Main Authors Near, JA, Mahler, H R
Format Journal Article
LanguageEnglish
Published 01.01.2002
Subjects
cattle
dopamine
GTP
inhibition
spiperone
striate cortex
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Summary:The addition of GTP (50 mu M), MnCl sub(2) (1 mM) or EDTA (2 mM) had no effect on the affinity or capacity of bovine striatal plasma membranes for ( super(3)H)spiperone. However, GTP caused a decrease in the potency of dopamine as an inhibitor of ( super(3)H)spiperone binding under all conditions tested. Manganese enhanced the potency of dopamine both in the presence and absence of GTP, but NaCl (100 mM) had no effect. Neither manganese nor GTP caused any change in the affinity or capacity of bovine striatal membranes for the tritiated agonists dopamine, apomorphine or ADTN. In the light of this and other reports that GTP inhibits triated agonist binding in rat striatum, it is suggested that the absence of such inhibition in bovine striatal membranes may reflect a fundamental difference between the two species with regard to their receptors for dopamine agonists.
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ISSN:0364-3190