Induction and Regulation of Cellulase Synthesis in Trichoderma pseudokoningii Mutants EA sub(3)-867 and N sub(2)-78

• Published in: Enzyme and microbial technology Vol. 4; no. 1; p. 3
• Main Authors: Zhu, Y S, Wu, Y Q, Chen, W, Tan, C, Gao, J H, Fei, J X, Shih, C N
• Format: Journal Article
• Language: English
• Published: 01.01.1982
• ISSN: 0141-0229

Two mutants, EA sub(3)-867 and N sub(2)-78, with high cellulase yields were obtained from wild strains of Trichoderma pseudokoningii) Rifai, 1096 and Mo sub(3), respectively, by mutagenic treatments with a linear accelerator, super(60)Co, u.v., nitrosoguanidine (NTG) and diethylsulphate (DTS). The cellulase activities of these mutants in Koji extracts, shake flask culture filtrates, and enzyme preparations were markedly higher than those of their parents. The authors conclude that cellulase synthesis of the mutants is regulated by catabolite repression as well as by induction. The increase in cellulase production by both mutants results from changes in the regulatory systems for cellulase synthesis, i.e. the mutants showed higher sensitivity to inducer and lower susceptibility to catabolite repression than did the wild types.