Cyclic AMP-dependent and -independent regulation of cholesterol side chain cleavage cytochrome P-450 (P-450 sub(scc)) in rat ovarian granulosa cells and corporal lutea. cDNA and deduced amino acid sequence of rat P-450 sub(scc)

We report the isolating and sequencing of three cDNA clones encoding rat P-450 sub(scc), the nucleotide and protein sequences of which are highly homologous to those of bovine and human P-450 sub(scc), especially in the putative heme and steroid binding domains. We document that different molecular...

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Published inThe Journal of biological chemistry Vol. 264; no. 36; pp. 21934 - 21942
Main Authors Oonk, R B, Krasnow, J S, Beattie, W G, Richards, J S
Format Journal Article
LanguageEnglish
Published 01.01.1989
Subjects
corpus luteum
genes
granulosa cells
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Summary:We report the isolating and sequencing of three cDNA clones encoding rat P-450 sub(scc), the nucleotide and protein sequences of which are highly homologous to those of bovine and human P-450 sub(scc), especially in the putative heme and steroid binding domains. We document that different molecular mechanisms regulate P-450 sub(scc) in granulosa cells of preovulatory (PO) follicles prior to and after luteinization. Luteinizing hormone/human chorionic gonadotropin (LH/hCG) and cAMP are obligatory to induce P-450 sub(scc) mRNA in PO granulosa cells in vivo and in vitro. The data suggest that expression of the P-450 sub(scc) gene in rat ovarian follicular cells is regulated in a sequential manner by cAMP-dependent and cAMP-independent mechanisms associated with granulosa cells and luteal cells, respectively.
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ISSN:0021-9258