Ethoxylated alcohol (Neodol-12) and other surfactants in the assay of protein kinase C

Most commonly used surfactants were found to be inhibitors of partially purified rat brain protein kinase C at or above their critical micellar concentrations (CMC). These include sodium lauryl sulfate, deoxycholate, octyl glucoside, dodecyl trimethylammonium bromide, linear alkylbenzene sulfonate a...

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Published inBiochimica et biophysica acta, Protein structure and molecular enzymology Vol. 992; no. 3; pp. 362 - 368
Main Authors Abidi, T F, Faaland, CA, Scala, D D, Rhein, L D, Laskin, J D
Format Journal Article
LanguageEnglish
Published 01.01.1989
Subjects
alcohols
brain
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Summary:Most commonly used surfactants were found to be inhibitors of partially purified rat brain protein kinase C at or above their critical micellar concentrations (CMC). These include sodium lauryl sulfate, deoxycholate, octyl glucoside, dodecyl trimethylammonium bromide, linear alkylbenzene sulfonate and Triton X-100. Several detergents, including the nonionic surfactants digitonin and Neodol-12 (ethoxylated alcohol), did not inhibit protein kinase C activity, even at concentrations greater than their CMC, while the anionic surfactant, AEOS-12 (ethoxylated alcohol sulfate), inhibited enzyme activity only slightly (less than 8%). The results indicate that activity of protein kinase C can be modified by the conditions of the assay and by the detergents used to extract the enzyme.
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ISSN:0167-4838