Human teratocarcinoma cells express functional insulin-like growth factor 1 receptors

• Published in: Experimental cell research Vol. 184; no. 2; pp. 427 - 439
• Main Authors: Weima, S M, Stet, L H, Van Rooijen, MA, Van Buul-Offers, SC, Van Zoelen, EJJ, De Laat, SW, Mummery, CL
• Format: Journal Article
• Language: English
• Published: 01.01.1989
• Subjects: insulin-like growth factor I; teratocarcinoma
• ISSN: 0014-4827

Using iodinated insulin-like growth factors (IGFs) the authors have detected receptors for IGF-I at the cell surface of the clonally derived human embryonal carcinoma cell line Tera 2 clone 13. Affinity crosslinking of IGFs to Tera 2 clone 13-derived membrane preparations revealed the presence of proteins with features of both type-I and type-II IGF receptors. Treatment of Tera 2 clone 13 cells with retinoic acid to induce differentiation results in an increased number of cell surface receptors, apparently without altering the ratio of type-I and type-II receptors. In addition, Tera 2 clone 13 IGF-I receptors catalyze (auto)phosphorylation at tyrosine upon IGF-I and insulin binding. These findings suggest that type-I IGF receptors might be involved in mediating the effects of IGFs and insulin upon the proliferation of Tera 2 clone 13 cells.