The 56 kDa protein of human genital skin fibroblasts is identical to that radiolabelled by ( super(3)H)dihydrotestosterone 17 beta -bromoacetate

Analysis of soluble proteins from human genital skin fibroblasts by two-dimensional polyacrylamide gel electrophoresis reveals an abundant protein doublet of mol. wt 56,000 with isoelectric points (pI) of 6.7 and 6.5. This protein is absent in non-genital skin fibroblasts as well as in genital skin...

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Published inJournal of steroid biochemistry Vol. 33; no. 3; pp. 389 - 394
Main Authors Belsham, D D, Rosenmann, E, Pereira, F A, Williams, S G, Turney, M K, Kovacs, W J, Faber, LE, Wrogemann, K
Format Journal Article
LanguageEnglish
Published 01.01.1989
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Abstract Analysis of soluble proteins from human genital skin fibroblasts by two-dimensional polyacrylamide gel electrophoresis reveals an abundant protein doublet of mol. wt 56,000 with isoelectric points (pI) of 6.7 and 6.5. This protein is absent in non-genital skin fibroblasts as well as in genital skin fibroblasts of most patients with complete forms of androgen insensitivity. The protein specifically binds androgen. A protein of similar estimated molecular weight (58,000) from human genital skin fibroblasts has recently been found to be covalently radiolabelled by the affinity ligand dihydrotestosterone 17 beta -bromoacetate (DHT-DA).
AbstractList Analysis of soluble proteins from human genital skin fibroblasts by two-dimensional polyacrylamide gel electrophoresis reveals an abundant protein doublet of mol. wt 56,000 with isoelectric points (pI) of 6.7 and 6.5. This protein is absent in non-genital skin fibroblasts as well as in genital skin fibroblasts of most patients with complete forms of androgen insensitivity. The protein specifically binds androgen. A protein of similar estimated molecular weight (58,000) from human genital skin fibroblasts has recently been found to be covalently radiolabelled by the affinity ligand dihydrotestosterone 17 beta -bromoacetate (DHT-DA).
Author Turney, M K
Rosenmann, E
Pereira, F A
Belsham, D D
Williams, S G
Kovacs, W J
Wrogemann, K
Faber, LE
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Title The 56 kDa protein of human genital skin fibroblasts is identical to that radiolabelled by ( super(3)H)dihydrotestosterone 17 beta -bromoacetate
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