The 56 kDa protein of human genital skin fibroblasts is identical to that radiolabelled by ( super(3)H)dihydrotestosterone 17 beta -bromoacetate
Analysis of soluble proteins from human genital skin fibroblasts by two-dimensional polyacrylamide gel electrophoresis reveals an abundant protein doublet of mol. wt 56,000 with isoelectric points (pI) of 6.7 and 6.5. This protein is absent in non-genital skin fibroblasts as well as in genital skin...
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Published in | Journal of steroid biochemistry Vol. 33; no. 3; pp. 389 - 394 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.01.1989
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Online Access | Get full text |
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Summary: | Analysis of soluble proteins from human genital skin fibroblasts by two-dimensional polyacrylamide gel electrophoresis reveals an abundant protein doublet of mol. wt 56,000 with isoelectric points (pI) of 6.7 and 6.5. This protein is absent in non-genital skin fibroblasts as well as in genital skin fibroblasts of most patients with complete forms of androgen insensitivity. The protein specifically binds androgen. A protein of similar estimated molecular weight (58,000) from human genital skin fibroblasts has recently been found to be covalently radiolabelled by the affinity ligand dihydrotestosterone 17 beta -bromoacetate (DHT-DA). |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
ISSN: | 0022-4731 |