Transformation of leukotriene A sub(4) to lipoxins by rat kidney mesangial cell
Incubation of rat mesangial cells with leukotriene A sub(4) in the presence of calcium ionophore A23187 led to a substrate dependent formation of lipoxin and its isomers. The major metabolite coeluted with authentic lipoxin A sub(4) (LXA sub(4)) and lipoxin B sub(4) (LXB sub(4)) in RP-HPLC system, a...
Saved in:
Published in | Biochemical and biophysical research communications Vol. 162; no. 2; pp. 626 - 633 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
01.01.1989
|
Online Access | Get full text |
Cover
Loading…
Summary: | Incubation of rat mesangial cells with leukotriene A sub(4) in the presence of calcium ionophore A23187 led to a substrate dependent formation of lipoxin and its isomers. The major metabolite coeluted with authentic lipoxin A sub(4) (LXA sub(4)) and lipoxin B sub(4) (LXB sub(4)) in RP-HPLC system, and possessed a characteristic U.V. spectrum and C-value which were identical to authentic standards. The generation of LXA sub(4) from LTA sub(4) in mesangial cells suggested that there is an active 15- or 12- lipoxygenase activity in the kidney. The production of LX may play an important role in the regulation of renal function and the response to inflammatory stimuli. |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
ISSN: | 0006-291X |