Transformation of leukotriene A sub(4) to lipoxins by rat kidney mesangial cell

Incubation of rat mesangial cells with leukotriene A sub(4) in the presence of calcium ionophore A23187 led to a substrate dependent formation of lipoxin and its isomers. The major metabolite coeluted with authentic lipoxin A sub(4) (LXA sub(4)) and lipoxin B sub(4) (LXB sub(4)) in RP-HPLC system, a...

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Published inBiochemical and biophysical research communications Vol. 162; no. 2; pp. 626 - 633
Main Authors Garrick, R, Shen, Shu-Ying, Ogunc, S, Wong, PY-K
Format Journal Article
LanguageEnglish
Published 01.01.1989
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Summary:Incubation of rat mesangial cells with leukotriene A sub(4) in the presence of calcium ionophore A23187 led to a substrate dependent formation of lipoxin and its isomers. The major metabolite coeluted with authentic lipoxin A sub(4) (LXA sub(4)) and lipoxin B sub(4) (LXB sub(4)) in RP-HPLC system, and possessed a characteristic U.V. spectrum and C-value which were identical to authentic standards. The generation of LXA sub(4) from LTA sub(4) in mesangial cells suggested that there is an active 15- or 12- lipoxygenase activity in the kidney. The production of LX may play an important role in the regulation of renal function and the response to inflammatory stimuli.
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ISSN:0006-291X