Ha-ras activates the Na super(+)/H super(+) antiporter by a protein kinase C-independent mechanism

In quiescent Ha-ras -transfected NIH 3T3 cells, addition of serum growth factors, bombesin or 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to a dimethylamiloride-sensitive intracellular alkalinization, which can be inhibited by staurosporine, a potent inhibitor of protein kinase C. Expression of...

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Published inThe Journal of biological chemistry Vol. 264; no. 20; pp. 11839 - 11842
Main Authors Maly, K, Ueberall, F, Loferer, H, Doppler, W, Oberhuber, H, Groner, B, Grunicke, H H
Format Journal Article
LanguageEnglish
Published 01.01.1989
Subjects
3T3 cells
Ha-ras
hydrogen
sodium
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Summary:In quiescent Ha-ras -transfected NIH 3T3 cells, addition of serum growth factors, bombesin or 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to a dimethylamiloride-sensitive intracellular alkalinization, which can be inhibited by staurosporine, a potent inhibitor of protein kinase C. Expression of the transforming Ha-ras gene causes a growth factor-independent increase in cytoplasmic pH. This Ha-ras -induced alkalizination is sensitive to dimethylamiloride but is not affected by staurosporine concentrations which prevent the pH response after addition of growth factors or TPA.
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SourceType-Scholarly Journals-1
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ISSN:0021-9258