Facilitated micromethod for measurement of metabolically generated super(14)CO sub(2), with application to measurement of ornithine decarboxylase

A modified microassay for the determination of metabolically generated super(14)CO sub(2) is described and is applied to the measurement of ornithine decarboxylase in animal tissue preparations. Recovery of evolved super(14)CO sub(2) was greater (97.5 vs 91.5%) and variation between replicate sample...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 178; no. 1; pp. 52 - 56
Main Authors Gaines, D W, Friedman, L, Braunberg, R C
Format Journal Article
LanguageEnglish
Published 01.01.1989
Subjects
ornithine decarboxylase
rats
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Summary:A modified microassay for the determination of metabolically generated super(14)CO sub(2) is described and is applied to the measurement of ornithine decarboxylase in animal tissue preparations. Recovery of evolved super(14)CO sub(2) was greater (97.5 vs 91.5%) and variation between replicate samples was less (coefficient of variation 2.7 vs 8.5%) when the modified microassay was compared with an assay system that requires removal of the screw cap from the vial before acid injection.
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ISSN:0003-2697