Resolution of the two components of macrophage inflammatory protein 1, and cloning and characterization of one of those components, macrophage inflammatory proteins 1 beta

A number of macrophage-derived mediators have been implicated in the vascular changes of inflammation. The authors recently reported the isolation of a novel monokine, macrophage inflammatory protein 1 (MIP-1), which causes local inflammatory responses in vivo, and induces superoxide production by n...

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Published inThe Journal of experimental medicine Vol. 168; no. 6; pp. 2251 - 2259
Main Authors Sherry, B, Tekamp-Olson, P, Gallegos, C, Bauer, D, Davatelis, G, Wolpe, S D, Masiarz, F, Coit, D, Cerami, A
Format Journal Article
LanguageEnglish
Published 01.01.1988
Subjects
genes
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Summary:A number of macrophage-derived mediators have been implicated in the vascular changes of inflammation. The authors recently reported the isolation of a novel monokine, macrophage inflammatory protein 1 (MIP-1), which causes local inflammatory responses in vivo, and induces superoxide production by neutrophils in vitro. The authors report here the resolution of MIP-1 into component peptides by SDS-hydroxylapatite chromatography, and compare the NH sub(2)-terminal sequences of the two peptides, now referred to as MIP-1 alpha and MIP-1 beta . A synthetic oligonucleotide probe pool corresponding to the NH sub(2)-terminal amino acid sequence of MIP-1 beta was used to isolate a cDNA clone containing its coding sequence.
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ISSN:0022-1007