Field testing and comparative evaluation of rapid, visually read screening assays for antibody to human immunodeficiency virus

Five rapid, visually read assays for immunodeficiency virus (HIV) were evaluated on fresh serum samples from 4000 prospective blood donors at Mama Yemo Hospital, Kinshasa, Zaire. The sensitivity of the assays, based on 214 specimens positive by western blot, ranged from 84 multiplied by 6% to 99 mul...

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Published inThe Lancet (North American edition) Vol. 1; no. 8638; pp. 580 - 584
Main Authors Spielberg, F, Kabeya, C M, Ryder, R W, Kifuani, N K, Harris, J, Bender, T R, Heyward, W L, Quinn, T C
Format Journal Article
LanguageEnglish
Published 01.01.1989
Subjects
human immunodeficiency virus
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Summary:Five rapid, visually read assays for immunodeficiency virus (HIV) were evaluated on fresh serum samples from 4000 prospective blood donors at Mama Yemo Hospital, Kinshasa, Zaire. The sensitivity of the assays, based on 214 specimens positive by western blot, ranged from 84 multiplied by 6% to 99 multiplied by 1%. The specificity, based on 3664 samples negative by enzyme-linked immunosorbent assay (ELISA) or western blot, ranged from 92 multiplied by 7% to 98 multiplied by 85. Three readers scored each test result independently; disagreement about test interpretation occurred in 1 multiplied by 2-8 multiplied by 3% of the specimens. There was no correlation between assay performance and assay principle (agglutination or dot immunobinding) or antigen source (viral lysate or recombinant).
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ISSN:0099-5355