Evidence for Cl super(-) exchangers in perfused Carcinus) gills

• Published in: Comparative biochemistry and physiology. A, Comparative physiology Vol. 92A; no. 3; pp. 415 - 420
• Main Author: Lucu, C
• Format: Journal Article
• Language: English
• Published: 01.01.1989
• Subjects: Brackish; Carcinus; Marine
• ISSN: 0300-9629

The effects of inhibitors and ion substitution on TBP (transbranchial potentials) and unidirectional super(22)Na and super(36)Cl fluxes from the bathing medium across the apical and basolateral sides into the perfusion solution J sub(a arrow right b) of isolated Carcinus gill epithelia were studied. Identical, diluted sea-water (DSW) and artificial solutions were used in the bathing solution and perfusate (202 plus or minus 5mM Na). Externally applied bumetanide (10 super(-3) M) did not affect super(36)Cl and super(22)Na fluxes. 10 super(-3) M 4-acetamido-4 isothiocyanostilbene 2,2 disulphonic acid (SITS) in the bathing solution and on both sides (apical and basolateral surfaces) effected a rapid, reversible and statistically significant inhibition of super(36)Cl J sub(a arrow right b) fluxes of 27.6 and 39.4%, respectively. On the basis of the experimental evidence described, it is suggested that there is a Cl super(-)/HCO sub(3) (or OH super(-)) antiporter located on the apical side of the gill surface.