Iodination of (Tyr super(11))somatostatin yields a super high affinity ligand for somatostatin receptors in GH sub(4)C sub(1) pituitary cells

GH sub(4)C1) cells are a clonal strain of rat pituitary tumor cells which contain high affinity receptors for the inhibitory neuropeptide somatostatin (SRIF). In contrast to other peptides that bind to specific receptors on these cells, receptor-bound ( super(125)I-Tyr super(1))SRIF does not undergo...

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Published inMolecular pharmacology Vol. 34; no. 5; pp. 651 - 658
Main Authors Presky, D H, Schonbrunn, A
Format Journal Article
LanguageEnglish
Published 01.01.1988
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Summary:GH sub(4)C1) cells are a clonal strain of rat pituitary tumor cells which contain high affinity receptors for the inhibitory neuropeptide somatostatin (SRIF). In contrast to other peptides that bind to specific receptors on these cells, receptor-bound ( super(125)I-Tyr super(1))SRIF does not undergo rapid endocytosis. The authors characterize the binding, biological activity and receptor-mediated degradation of ( super(125)I-Tyr super(11))SRIF, a SRIF analog that is radiolabeled in the center of the molecule. The data demonstrate that ( super(125)I-Tyr super(11))SRIF is a better radioanalog than ( super(125)I-Tyr super(11))SRIF for binding studies with intact cells because of its higher affinity for the SRIF receptor.
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ISSN:0026-895X