A two-dimensional super(1)H-NMR study of the dam) methylase site: Comparison between the hemimethylated GATC sequence, its unmethylated analogue and a hemimethylated CATG sequence. The sequence dependence of methylation upon base-pair lifetimes

The authors report two-dimensional NOE (NOESY) spectra on the sequence d(GCGATCATGG) multiplied by d(CCATGATCGC) which contains the unmethylated dam site. As expected the DNA adopts a B-form conformation but appears to be distorted at the TG step of the second strand. This distorsion, probably bendi...

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Published inEuropean journal of biochemistry Vol. 167; no. 3; pp. 397 - 404
Main Authors Fazakerley, G V, Quignard, E, Teoule, R, Guy, A, Guschlbauer, W
Format Journal Article
LanguageEnglish
Published 01.01.1987
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Summary:The authors report two-dimensional NOE (NOESY) spectra on the sequence d(GCGATCATGG) multiplied by d(CCATGATCGC) which contains the unmethylated dam site. As expected the DNA adopts a B-form conformation but appears to be distorted at the TG step of the second strand. This distorsion, probably bending, is not seen on the opposite strand. When the first strand is methylated on adenine in the GATC or CATG sequence the NOESY spectra indicate little or no change in the conformation. However the single strand-duplex exchange is slowed down to the slow-exchange region on a proton NMR time scale. They have assigned the exchangeable imino and cytidine amino resonances of the three duplexes.
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ISSN:0014-2956