Inhibition of mitochondrial F sub(1)-ATPase activity by an anti- alpha subunit monoclonal antibody which modifies interactions between catalytic and regulatory sites

A monoclonal antibody, 7B3, specific to the alpha subunit of the mitochondrial ATPase-ATP synthase inhibited the rate of ATP hydrolysis by either soluble F sub(1) or electron transport particles up to a maximum of 75%. However, 7B3 did not modify the rate of ITP hydrolysis. The use of this monoclona...

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Bibliographic Details
Published inThe Journal of biological chemistry Vol. 264; no. 3; pp. 1361 - 1367
Main Authors Moradi-Ameli, M, Julliard, J H, Godinot, C
Format Journal Article
LanguageEnglish
Published 01.01.1989
Subjects
adenosinetriphosphatase
heart
mitochondria
monoclonal antibodies
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Summary:A monoclonal antibody, 7B3, specific to the alpha subunit of the mitochondrial ATPase-ATP synthase inhibited the rate of ATP hydrolysis by either soluble F sub(1) or electron transport particles up to a maximum of 75%. However, 7B3 did not modify the rate of ITP hydrolysis. The use of this monoclonal antibody demonstrates for the first time in mammalian F sub(1) the role of the conformation of the alpha subunit in the regulation of the ATPase activity.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ObjectType-Feature-1
ISSN:0021-9258