A monoclonal anti-type II collagen antibody with cross-reactive anti-Ig activity specific for the F(ab') sub(2) fragment

An IgG2a hybridoma antibody (BC-10) was obtained by a myeloma fusion with lymphocytes from B10.RIII mice immunized against native bovine type II collagen. This anti-collagen monoclonal exhibited extensive cross-reactivity with several type II collagen species. BC-10 was found to have self-associatin...

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Bibliographic Details
Published inThe Journal of immunology (1950) Vol. 141; no. 11; pp. 3819 - 3822
Main Authors Punjabi, C J, Wood, D D, Wooley, PH
Format Journal Article
LanguageEnglish
Published 01.01.1988
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Summary:An IgG2a hybridoma antibody (BC-10) was obtained by a myeloma fusion with lymphocytes from B10.RIII mice immunized against native bovine type II collagen. This anti-collagen monoclonal exhibited extensive cross-reactivity with several type II collagen species. BC-10 was found to have self-associating properties, but not the specificity of a typical IgG rheumatoid factor, inasmuch as this mAb bound to F(ab') sub(2) fragments of itself and of normal mouse IgG. Joint inflammation and histopathologic features consistent with arthritis were observed in mice injected with the BC-10 hybridoma.
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ISSN:0022-1767