Two monoclonal rat antibodies with specificity for the beta -chain variable region V sub( beta )6 of the murine T-cell receptor

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 85; no. 20; pp. 7695 - 7698
• Main Authors: Payne, J, Huber, B T, Cannon, NA, Schneider, R, Schilham, M W, Acha-Orbea, H, McDonald, H R, Hengartner, H
• Format: Journal Article
• Language: English
• Published: 01.01.1988
• ISSN: 0027-8424

Two rat monoclonal antibodies (mAbs), 44-22-1 and 46-6B5, which recognize an alloreactive cytotoxic clone, 3F9, have been further tested on a panel of T hybridomas and cytotoxic T-cell clones for binding and functional activities. The mAbs recognized only those cells sharing the expression of the T-cell receptor beta -chain variable region gene V sub( beta )6 with 3F9. All V sub( beta )6 super(+) cells were activated by these mAbs under cross-linking conditions and their antigen-specific activation was blocked by soluble mAb. Furthermore, depletion of 46-6B5 super(+) normal lymph node T cells eliminated all cells expressing the epitope recognized by 44-22-1 and V sub( beta )6 mRNA.