Additional RNA polymerase I initiation site within the nonstranscribed spacer region of the rat rRNA gene

The authors identified and characterized an additional promoter within the nontranscribed spacer (NTS) of the rat ribosomal gene repeat that is capable of supporting initiation of transcription by RNA polymerase I in vitro. Within this promoter there is a sequence of 13 nucleotides which is 100% hom...

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Published inMolecular and cellular biology Vol. 7; no. 7; pp. 2388 - 2396
Main Authors Cassidy, B G, Yang-Yen, Hsin-Fang, Rothblum, LI
Format Journal Article
LanguageEnglish
Published 01.01.1987
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Summary:The authors identified and characterized an additional promoter within the nontranscribed spacer (NTS) of the rat ribosomal gene repeat that is capable of supporting initiation of transcription by RNA polymerase I in vitro. Within this promoter there is a sequence of 13 nucleotides which is 100% homologous to nucleotides -18 to -6 (+1 being the first nucleotide of 45S rRNA) of the major promoter of 45S pre-rRNA and is located between nucleotides -731 and -719. To identify the exact location of the upstream initiation site, the RNA synthesized in vitro from this new promoter was gel isolated and subjected to fingerprint analysis, Southern hybridization, and reverse transcriptase elongation.
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SourceType-Scholarly Journals-1
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ISSN:0270-7306