Comparative study of ceruloplasmin biosynthesis in various cell-free systems

The peculiarities of the translation of rat liver ceruloplasmin were investigated in three cell-free system: wheat germ extract, a lysate of rabbit reticulocytes, and an extract of Zajdela ascites hepatoma cells. It was shown that in a reticulocyte lysate and in an extract of tumor cells, full-sized...

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Published inBiochemistry (Easton) Vol. 52; no. 1; pp. 26 - 32
Main Authors Neifakh, SA, Gaitskhoki, V S, Denezhkina, V V, Puchkova, LV, Timchenko, L T
Format Journal Article
LanguageEnglish
Published 01.01.1987
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Summary:The peculiarities of the translation of rat liver ceruloplasmin were investigated in three cell-free system: wheat germ extract, a lysate of rabbit reticulocytes, and an extract of Zajdela ascites hepatoma cells. It was shown that in a reticulocyte lysate and in an extract of tumor cells, full-sized translation products of CP-mRNA, which approached the molecular weight of the mature CP molecules (122-132 kilodaltons), are synthesized. In a wheat germ extract the NH sub(2)-terminal fragment of the CP molecule, with molecular weight 84 kilodaltons, is synthesized. When liver membrane fractions are added to wheat germ extract, translating CP-mRNA, there is a reconstruction of the proteolytic steps in the maturation of CP.
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SourceType-Scholarly Journals-1
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ISSN:0006-2960