In vitro mapping of super(1)H ultrashort T sub(2) and T sub(2) of porcine menisci

• Published in: NMR in biomedicine Vol. 26; no. 9; pp. 1167 - 1175
• Main Authors: Kirsch, S, Kreinest, M, Reisig, G, Schwarz, MLR, Strobel, P, Schad, L R
• Format: Journal Article
• Language: English
• Published: 01.09.2013
• Subjects: Collagen
• ISSN: 0952-3480; 1099-1492
• DOI: 10.1002/nbm.2931

In this study, mapping of ultrashort T sub(2) and T sub(2)* of acutely isolated porcine menisci at B sub(0)=9.4T was investigated. Maps of T sub(2) were measured from a slice through the pars intermedia with a spin echo-prepared two-dimensional ultrashort-TE T sub(2) mapping technique published previously. T sub(2)* mapping was performed by two-dimensional ultrashort-TE MRI with variable acquisition delay. The measured signal decays were fitted by monoexponential, biexponential and Gaussian-exponential fitting functions. The occurrence of Gaussian-like signal decays is outlined theoretically. The quality of the curve fits was visualized by mapping the value [delta] = abs(1 - chi super(2) sub(red)). For T sub(2)* mapping, the Gaussian-exponential fit showed the best performance, whereas the monoexponential and biexponential fits showed regionally high values of [delta] ([delta] > 20). Interpretation of the Gaussian-exponential parameter maps was found to be difficult, because a Gaussian signal component can be related to mesoscopic (collagen texture) or macroscopic (slice profile, shim, sample geometry) magnetic field inhomogeneities and/or residual super(1)H dipole-dipole couplings. It seems likely that an interplay of these effects yielded the observed signal decays. Modulation of the T sub(2)* signal decay caused by chemical shift was observed and addressed to fat protons by means of histology. In the T sub(2) measurements, no modulation of the signal decay was observed and the biexponential and Gaussian-exponential fits showed the best performance with comparable values of [delta]. Our results suggest that T sub(2) mapping provides the more robust method for the characterization of meniscal tissue by means of MRI relaxometry. However, mapping of ultrashort T sub(2), as performed in this study, is time consuming and provides less signal-to-noise ratio per time than the mapping of T sub(2)*. If T sub(2)* mapping is used, pixel-wise monitoring of the fitting quality based on reduced chi super(2) should be employed and great care should be taken when interpreting the parameter maps of the fits. Copyright copyright 2013 John Wiley & Sons, Ltd. Mapping of ultrashort T sub(2) and T sub(2)* of isolated porcine menisci was investigated. The signal decays were fitted by monoexponential (ME), biexponential (BIE) and Gaussian-exponential (GE) fitting functions. The occurrence of Gaussian-like T sub(2)* signal decays is theoretically outlined. The quality of the fit was pixel-wise visualized by mapping of [delta]=abs(1- chi super( 2) sub(red)). The GE and BIE fits showed the best performance. The results suggest that T sub(2) mapping is more robust than T sub(2)* mapping in the context of MRI relaxometry of menisci.